rabbit anti-mouse fsp1  (Merck & Co)

Journal: Mediators of Inflammation

Article Title: Resolution of PMA-Induced Skin Inflammation Involves Interaction of IFN- γ and ALOX15

doi: 10.1155/2013/930124

Figure Lengend Snippet: Neutralization of endogenous IFN- γ decreased the infiltration of inflammatory cells and the production of inflammatory cytokines in the skin. Mice were sacrificed 3 hours, 4 days, or 7 days after the third PMA treatment. (a) Skin sections were stained with anti-CD11b, anti-Gr1, or anti-FSP1 (red). Nucleoli were stained with DAPI (blue). Scale Bar, 50 μ m. (b) The expression levels of IL-6, TNF- α , MCP-1, IL-1 α , and IL-1 β of the skin from control group or anti-IFN- γ group were detected at indicated time points. * P < 0.05, compared to control group.

Article Snippet: For immunofluorescence analysis, sections were stained with rat anti-mouse CD11b (1 : 100; BD Pharmingen, USA), rat anti-mouse granulocyte differentiation antigen 1 (Gr1, 1 : 100; BD Pharmingen, USA), rabbit anti-mouse fibroblast-specific protein-1 (FSP1, 1 : 100; BD Pharmingen, USA), and subsequently rhodamine-labeled secondary antibody and counterstained with 4, 6-diamidino-2-phenylindole (DAPI; Sigma, USA).

Techniques: Neutralization, Staining, Expressing

Journal: Arthritis Research & Therapy

Article Title: Angiotensin II induces skin fibrosis: a novel mouse model of dermal fibrosis

doi: 10.1186/ar4028

Figure Lengend Snippet: Primers for quantitative real-time polymerase chain reaction

Article Snippet: EndoMT , Goat anti-mouse VE-cadherin Ab (Santa Cruz Biotechnology, Santa Cruz, CA); 1:300 , Rabbit anti-mouse FSP1 Ab (Abcam); 1:100 , Alexa fluor 488 donkey anti-goat IgG (Invitrogen); 1:1,000 , Alexa fluor 594 donkey anti-rabbit IgG (Invitrogen); 1:1,000.

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Angiotensin II induces skin fibrosis: a novel mouse model of dermal fibrosis

doi: 10.1186/ar4028

Figure Lengend Snippet: Primary and secondary antibodies for immunofluorescence staining

Article Snippet: EndoMT , Goat anti-mouse VE-cadherin Ab (Santa Cruz Biotechnology, Santa Cruz, CA); 1:300 , Rabbit anti-mouse FSP1 Ab (Abcam); 1:100 , Alexa fluor 488 donkey anti-goat IgG (Invitrogen); 1:1,000 , Alexa fluor 594 donkey anti-rabbit IgG (Invitrogen); 1:1,000.

Techniques: Immunofluorescence

Journal: Arthritis Research & Therapy

Article Title: Angiotensin II induces skin fibrosis: a novel mouse model of dermal fibrosis

doi: 10.1186/ar4028

Figure Lengend Snippet: Recruitment of fibrocytes after angiotensin II treatment . (A) Immunofluorescence staining of CD45 (red) and FSP1 (green) on cryosections of skin samples from PBS- and Ang II-treated mice. Arrows indicate CD45/FSP1 and CD45/P4H double-positive cells. The bar graphs represent quantification of percentage of total positive cells/HPF (high-power field) for CD45, FSP1, CD45/FSP1, and CD45/P4H in PBS- and Ang II-treated mice (* P ≤ 0.05). Representative images are shown from four animals per group. (B) Real-time PCR for MCP1 and FSP1 in PBS (white bars) and Ang II (black bars)-treated mice (* P ≤ 0.05).

Article Snippet: EndoMT , Goat anti-mouse VE-cadherin Ab (Santa Cruz Biotechnology, Santa Cruz, CA); 1:300 , Rabbit anti-mouse FSP1 Ab (Abcam); 1:100 , Alexa fluor 488 donkey anti-goat IgG (Invitrogen); 1:1,000 , Alexa fluor 594 donkey anti-rabbit IgG (Invitrogen); 1:1,000.

Techniques: Immunofluorescence, Staining, Real-time Polymerase Chain Reaction

Journal: Arthritis Research & Therapy

Article Title: Angiotensin II induces skin fibrosis: a novel mouse model of dermal fibrosis

doi: 10.1186/ar4028

Figure Lengend Snippet: Effect of angiotensin II on EndoMT . Immunofluorescence staining of VE-cadherin (green) and FSP1 (red) in skin samples from PBS- and Ang II-treated mice (left panel) and HDMECs treated with 1,000 ng/ml of Ang II and control (right panel). Arrows indicate Ve-cadherin/FSP1 double-positive cells. Representative images are shown from five animals per group.

Article Snippet: EndoMT , Goat anti-mouse VE-cadherin Ab (Santa Cruz Biotechnology, Santa Cruz, CA); 1:300 , Rabbit anti-mouse FSP1 Ab (Abcam); 1:100 , Alexa fluor 488 donkey anti-goat IgG (Invitrogen); 1:1,000 , Alexa fluor 594 donkey anti-rabbit IgG (Invitrogen); 1:1,000.

Techniques: Immunofluorescence, Staining, Control